UBE2T
HUABIO
catalog: HA721385

domestic rabbit monoclonal (JE65-79)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of UBE2T on different lysates with Rabbit anti-UBE2T antibody (HA721385) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: MCF7 cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Lane 5: HEK-293 cell lysate (15 µg/Lane) Lane 6: Jurkat cell lysate (15 µg/Lane) Lane 7: SKOV-3 cell lysate (15 µg/Lane) Lane 8: SH-SY5Y cell lysate (15 µg/Lane) Lane 9: HepG2 cell lysate (15 µg/Lane) Lane 10: Mouse testis tissue lysate (30 µg/Lane) Lane 11: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 22.5 kDa Observed band size: 22.5 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721385) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling UBE2T with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier