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- mouse monoclonal (3.00E+11)
reactivity: human, rat
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 5 - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 2

Western Blot: Twist-1 Antibody [NB120-49254] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%
quantity: 100 ul
price: 409 USD
to the supplier - mouse monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry - domestic rabbit monoclonal (PSH11-98)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Twist1 on different lysates with Rabbit anti-Twist1 antibody (HA723411) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: A375 cell lysate Lane 3: PC-3M cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: E14.0 mouse embryonic brain tissue lysate Lane 8: Rat embryo tissue lysate Lane 9: LLC cell lysate Lane 10: Mouse embryo tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 24 kDa Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH11-98)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Twist1 on different lysates with Rabbit anti-Twist1 antibody (HA751431) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: A375 cell lysate Lane 3: PC-3M cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: E14.0 mouse embryonic brain tissue lysate Lane 8: Rat embryo tissue lysate Lane 9: LLC cell lysate Lane 10: Mouse embryo tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 24 kDa Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751431) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Twist1
- synonym:Twist
- description:twist family bHLH transcription factor 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Twist1 antibody
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