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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
citations: 1
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200).3,4. Anti-TSH Receptor (TSHR) (extracellular) Antibody, preincubated with TSH Receptor/TSHR (extracellular) Blocking Peptide (#BLP-TR006).
Expression of TSH Receptor in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in the pyramidal layer (P, arrows) and in mossy fiber terminal region (MF). B. Pre-incubation of the antibody with TSH Receptor (TSHR) (extracellular) Blocking Peptide (BLP-TR006), suppressed staining. Cell nuclei are stained with DAPI (blue).
Peptide (C)KLPKNINGTDPIQK corresponding to amino acid residues 487-500 of human ANO6 (Accession Q4KMQ2). 2nd extracellular loop.
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry
Expression of TSH receptor in mouse brain - Immunohistochemical staining of TSHR in mouse brain sections using Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody (#ATR-006-AG), (1:60). A. In the cerebellum, TSHR staining (green) appears in the molecular layer (MOL) and purkinje (P) layer. B. In the hippocampus, staining appears in CA3 pyramidal layer (P), (arrows). Nuclei are stained using DAPI (blue).
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to the supplier - domestic rabbit polyclonal
reactivity: human, rat
application: western blot
Figure 1. Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (MBS1750438). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
quantity: 0.1 mg
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
Western blot analysis of extracts of various cell lines, using TSHR antibody.
quantity: 0.06 mL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: flow cytometry, immunohistochemistry - paraffin section
gene information - rat Tshr
- synonym:TSHRA
- description:thyroid stimulating hormone receptor
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Tshr antibody
- Tshr protein
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