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- mouse monoclonal (S74)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 6
Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (SMC-316). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (SMC-316) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3 - mouse monoclonal (S74)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1 - domestic rabbit monoclonal (JE38-36)
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of TRPM7 on different lysates with Rabbit anti-TRPM7 antibody (HA721501) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 213 kDa Observed band size: 250 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa cells labeling TRPM7. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721501, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Trpm7
- synonym:Chak; LTrpC-7; Ltrpc7; Trp-plik
- description:transient receptor potential cation channel, subfamily M, member 7
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product type
- Trpm7 antibody
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