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- domestic rabbit monoclonal (JE63-45)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: JAR cell lysate, 10 µg/Lane Lane 2: HepG2 cell lysate, 10 µg/Lane Lane 3: 293T cell lysate, 10 µg/Lane Lane 4: NIH/3T3 cell lysate, 10 µg/Lane Lane 5: Mouse colon tissue lysate, 20 µg/Lane Predicted band size: 68 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Cip4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 80 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: PC-12 cell lysate, 10 µg/Lane Lane 2: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 68 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (21/CIP4)
reactivity: human, mouse, rat, dogs
application: western blot, immunocytochemistry, immunoprecipitation
citations: 2
gene information - rat Trip10
- synonym:Cip4; STP
- description:thyroid hormone receptor interactor 10
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Trip10 antibody
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