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- domestic rabbit monoclonal (PSH03-38)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of TOM1 on different lysates with Rabbit anti-TOM1 antibody (HA721983) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: U-2 OS cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: JAR cell lysate (20 µg/Lane) Lane 8: THP-1 cell lysate (20 µg/Lane) Lane 9: C2C12 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse heart tissue lysate (40 µg/Lane) Lane 12: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 13: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721983) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of paraffin-embedded human stomach carcinoma tissue labeling TOM1 with Rabbit anti-TOM1 antibody (HA721983) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721983, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis of TOM1 on different lysates with Rabbit anti-TOM1 antibody (HA721983) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TOM1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721983) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH03-38)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of TOM1 on different lysates with Rabbit anti-TOM1 antibody (HA750872) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: U-2 OS cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: JAR cell lysate (20 µg/Lane) Lane 8: THP-1 cell lysate (20 µg/Lane) Lane 9: C2C12 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse heart tissue lysate (40 µg/Lane) Lane 12: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 13: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750872) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of paraffin-embedded human stomach carcinoma tissue labeling TOM1 with Rabbit anti-TOM1 antibody (HA750872) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750872, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis of TOM1 on different lysates with Rabbit anti-TOM1 antibody (HA750872) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TOM1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750872) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Tom1
- description:target of myb1 membrane trafficking protein
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
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product type
- Tom1 antibody
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