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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, enzyme immunoassay - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay - domestic rabbit monoclonal (PSH0-53)
reactivity: human, rat
application: western blot
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/1,000 dilution. Lane 1: PC-12 whole cell lysate Lane 2: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate Lane 3: PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Tankyrase 2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 90 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH0-53)
reactivity: human, rat
application: western blot 
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/1,000 dilution. Lane 1: PC-12 whole cell lysate Lane 2: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate Lane 3: PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750630) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Tankyrase 2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 90 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750630) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Tnks2
- description:tankyrase 2
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product type
- Tnks2 antibody
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