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- domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
citations: 2 - domestic goat polyclonal
reactivity: human, rat
application: western blot, flow cytometry - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4): - 1,2. Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033), (1:400).3,4. Anti-TROY (TNFRSF19) (extracellular) Antibody, preincubated with TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033).
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to the supplier - domestic rabbit monoclonal (JE54-51)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of TNFRSF19 on different lysates with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/500 dilution. Lane 1: mouse testis tissue lysate(20 µg/Lane) Lane 2: rat large intestine tissue lysate (20 µg/Lane) Lane 3: A431 cell lysate (10 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 1 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-74) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-74) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-74) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Tnfrsf19
- synonym:RGD1564996
- description:TNF receptor superfamily member 19
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Tnfrsf19 antibody
- Tnfrsf19 ELISA/assay
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