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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (ER1803-48) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: A-172 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 30 kDa Exposure time: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (ER1803-48) at 1/5,000 dilution. Lane 1: K-562 cell lysate (no heat) Lane 2: HeLa cell lysate (hot lysis) Lane 3: PC-12 cell lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 30 kDa Exposure time: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-48) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
gene information - rat Tmem248
- description:transmembrane protein 248
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product type
- Tmem248 antibody
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