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- domestic rabbit monoclonal (JE55-73)
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Themis on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-20, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining of Themis in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Themis antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry - domestic sheep polyclonal
reactivity: mouse, rat
application: western blot 
Detection of Mouse and Rat Themis by Western Blot. Western blot shows lysates of mouse thymus tissue and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 1 ╡g/mL of Sheep Anti-Mouse/Rat Themis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6816) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Themis at approximately 73 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 449 USD
to the supplier
gene information - rat Themis
- synonym:RGD1560849
- description:thymocyte selection associated
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product type
- Themis antibody
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