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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of TCF21 on different lysates with Rabbit anti-TCF21 antibody (HA500114) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 20 kDa Observed band size: 26 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500114) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TCF21 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500114, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - rat Tcf21
- synonym:Capsulin; Msf-1; Pod1
- description:transcription factor 21
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- Tcf21 antibody
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