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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of TAB1 on different lysates with Rabbit anti-TAB1 antibody (HA500400) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TAB1 on mouse brain tissue lysates with Rabbit anti-TAB1 antibody (HA500400) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TAB1 on different lysates with Rabbit anti-TAB1 antibody (HA500400) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TAB1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500400) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier - mouse monoclonal (2A12)
reactivity: human, rat
application: western blot, ELISA, immunocytochemistry, proximity ligation assay
gene information - rat Tab1
- synonym:Map3k7ip1
- description:TGF-beta activated kinase 1/MAP3K7 binding protein 1
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Tab1 antibody
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