Anti-LAT1 Antibody
MyBioSource
catalog: MBS8291984

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of LAT1 expression in MCF7 (A), HepG2 (B), mouse brain (C), rat liver (D) whole cell lysates.
quantity: 0.05 mL
price:
to the supplier

Anti-LAT1 (SLC7A5) Antibody
Alomone Labs
catalog: ANT-105

domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of rat placenta lysate (lanes 1 and 4), mouse testes lysate (lanes 2 and 5) and rat skeletal muscle membrane (lanes 3 and 6): - 1-3. Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200).4-6. Anti-LAT1 (SLC7A5) Antibody, preincubated with LAT1/SLC7A5 Blocking Peptide (#BLP-NT105).

Expression of LAT1/SLC7A5 in rat dorsal root ganglion.  - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. LAT1 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with LAT1 (SLC7A5) Blocking Peptide (#BLP-NT105), suppressed staining. Cell nuclei are stained with DAPI (blue).
quantity:
price:
to the supplier

SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
Novus Biologicals
catalog: NBP3-25549

domestic rabbit monoclonal (HL2353)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
quantity: 100 ul
price: 519 USD
to the supplier

SLC7A5 / LAT1
HUABIO
catalog: HA723086

domestic rabbit monoclonal (PSH09-28)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of SLC7A5 / LAT1 on different lysates with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: LNCaP cell lysate (negative) (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 37 kDa Exposure time: Lane 1-2: 1 minute; Lane 3-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723086) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 (positive) and LNCaP (negative) labeling SLC7A5 / LAT1 with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723086) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

SLC7A5 / LAT1
HUABIO
catalog: HA751282

domestic rabbit monoclonal (PSH09-28)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of SLC7A5 / LAT1 on different lysates with Rabbit anti-SLC7A5 / LAT1 antibody (HA751282) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: LNCaP cell lysate (negative) (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 37 kDa Exposure time: Lane 1-2: 1 minute; Lane 3-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751282) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 (positive) and LNCaP (negative) labeling SLC7A5 / LAT1 with Rabbit anti-SLC7A5 / LAT1 antibody (HA751282) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLC7A5 / LAT1 antibody (HA751282) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SLC7A5 / LAT1 antibody (HA751282) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751282) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier