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- domestic rabbit monoclonal (JG35-72)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of hnRNP Q on different lysates with Rabbit anti-hnRNP Q antibody (ET7108-17) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of hnRNP Q on rat brain tissue lysates with Rabbit anti-hnRNP Q antibody (ET7108-17) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-17) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ICC staining of hnRNP Q in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-17, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (D18B2)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
citations: 1
gene information - rat Syncrip
- synonym:Ab2-339; hnRNP Q
- description:synaptotagmin binding, cytoplasmic RNA interacting protein
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product type
- Syncrip antibody
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