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- domestic rabbit monoclonal
reactivity: mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section - domestic rabbit monoclonal (JG95-31)
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of SUN1 on different lysates with Rabbit anti-SUN1 antibody (ET7108-91) at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 90/100 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-91) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling SUN1 with Rabbit anti-SUN1 antibody (ET7108-91) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SUN1 antibody (ET7108-91) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Western blot analysis of SUN1 on rat kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Sun1
- synonym:Unc84a
- description:Sad1 and UNC84 domain containing 1
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product type
- Sun1 antibody
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