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- domestic rabbit monoclonal (JF0884)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of STAT2 on different lysates with Rabbit anti-STAT2 antibody (ET1702-18) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Lane 3: Ramos cell lysate (40 µg/Lane) Predicted band size: 98 kDa Observed band size: 105 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-18) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of STAT2 on different lysates with Rabbit anti-STAT2 antibody (ET1702-18) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A431 cells labeling STAT2 with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-STAT2 antibody (ET1702-18) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (4A5P8)
reactivity: human, rat
application: western blot - domestic rabbit polyclonal
reactivity: human, rat, bovine
modification: phosphorylated
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
citations: 3
gene information - rat Stat2
- description:signal transducer and activator of transcription 2
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Stat2 antibody
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