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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry - domestic rabbit monoclonal (PSH15-61)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA723768) at 1/5,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300n/mL BFA for 3 hours cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HepG2 treated with 10ng/mL BFA for 1.5 hours cell lysate (20 µg/Lane) Lane 5: MOLT-4 cell lysate (low expression) (20 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723768) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA723768) at 1/5,000 dilution. Lane 1: PC-12 cell lysate (40 µg/Lane) Lane 2: PC-12 starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate (40 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723768) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Acid sphingomyelinase on RAW264.7 cell lysates with Rabbit anti-Acid sphingomyelinase antibody (HA723768) at 1/2,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723768) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH15-61)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/5,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300n/mL BFA for 3 hours cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HepG2 treated with 10ng/mL BFA for 1.5 hours cell lysate (20 µg/Lane) Lane 5: MOLT-4 cell lysate (low expression) (20 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/5,000 dilution. Lane 1: PC-12 cell lysate (40 µg/Lane) Lane 2: PC-12 starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate (40 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Acid sphingomyelinase on RAW264.7 cell lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/2,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Smpd1
- description:sphingomyelin phosphodiesterase 1
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- Smpd1 antibody
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