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- mouse monoclonal (62-2E8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation
citations: 4 - mouse monoclonal (62-2E8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 3
Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.
quantity: 0.1 ml (also 0.025 ml)
price: 409 USD
to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of human SH-5YSY neuroblastoma cell lysate (lanes 1 and 4), rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysates: - 1-3. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (#ACT-001), (1:200).4-6. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody, preincubated with Choline Transporter/SLC5A7 (extracellular) Blocking Peptide (#BLP-CT001).
Expression of Choline transporter in rat striatum - Immunohistochemical staining of rat frozen brain sections using Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (#ACT-001) (1:200). A. CHT expression (green) is detected in the matrix (M) but not in the patches (P). B. DAPI (blue) staining provides general cellular staining. C. merge of A and B shows that CHT appears in a subset of striatal neurons (arrows).
Expression of Choline transporter in rat PC12 cells - Cell surface detection ofCHT in live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Choline Transporter (SLC5A7) (extracellular) Antibody(#ACT-001) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of the two images.
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gene information - rat Slc5a7
- synonym:Cht1
- description:solute carrier family 5 member 7
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Slc5a7 antibody
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