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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of AE 1 on different lysates with Rabbit anti-AE 1 antibody (HA500323) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: Rat kidney tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AE 1 antibody (HA500323) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500323) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AE 1 antibody (HA500323) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500323) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section 
Anti- Band 3 Picoband antibody, Western blotting. All lanes: Anti Band 3 at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Rat Kidney Tissue Lysate at 50ug. Lane 3: Rat Liver Tissue Lysate at 50ug. Lane 4: Rat Spleen Tissue Lysate at 50ug. Lane 5: Human Placenta Tissue Lysate at 50ug. Predicted bind size: 102KD. Observed bind size: 102KD
Anti- Band 3 Picoband antibody, PB9437,IHC(P). IHC(P): Rat Kidney Tissue
Anti- Band 3 Picoband antibody,IHC(P). IHC(P): Human Placenta Tissue
quantity: 0.1 mg
price:
to the supplier
gene information - rat Slc4a1
- description:solute carrier family 4 member 1 (Diego blood group)
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Slc4a1 antibody
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