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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry - domestic rabbit monoclonal (JE58-29)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of SLC27A4 on different lysates with Rabbit anti-SLC27A4 antibody (HA720001) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SLC27A4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720001) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SLC27A4 on different lysates with Rabbit anti-SLC27A4 antibody (HA720001) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: Mouse colon tissue lysate (40 µg/Lane) Lane 3: Mouse pancreas tissue lysate (40 µg/Lane) Lane 4: Rat colon tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 72 kDa Observed band size: 70 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720001) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SLC27A4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720001, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - rat Slc27a4
- synonym:Fatp4
- description:solute carrier family 27 member 4
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product type
- Slc27a4 antibody
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