SEC24D
HUABIO
catalog: HA721902

domestic rabbit monoclonal (JE65-27)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of SEC24D on different lysates with Rabbit anti-SEC24D antibody (HA721902) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 113 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721902) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of SEC24D on different lysates with Rabbit anti-SEC24D antibody (HA721902) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si SEC24D cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 113 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721902) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling SEC24D with Rabbit anti-SEC24D antibody (HA721902) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SEC24D antibody (HA721902) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier

SEC24D Antibody (JE65-27)
Novus Biologicals
catalog: NBP3-32937

domestic rabbit monoclonal (JE65-27)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

SEC24D Antibody (JE65-27)
quantity: 100 ul
price: 449 USD
to the supplier

Anti-SEC24D antibody [EPR16089] - N-terminal
Abcam
catalog: ab191566

domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100µL, 1 mL
price:
to the supplier

Anti-SEC24D antibody [EPR16089] - BSA and Azide free
Abcam
catalog: ab251088

domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100µg, 1 mg
price:
to the supplier