Your Filters
product
- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot
citations: 1
Western blot analysis of rat heart (lanes 1 and 3), rat kidney (lanes 2 and 4), human Jurkat acute T cell leukemia (lanes 5 and 7) and mouse WEHI B cell lymphoma (lanes 6 and 8) lysates: - 1,2,5,6. Anti-S1PR3 (EDG3) (extracellular) Antibody (#ASR-013), (1:200).3,4,7,8. Anti-S1PR3 (EDG3) (extracellular) Antibody, preincubated with S1PR3/EDG3 (extracellular) Blocking Peptide (#BLP-SR013).
Peptide (C)DYVGKLAGRLRD corresponding to amino acid residues 23-34 of mouse S1PR3 (AccessionQ9Z0U9). Extracellular N-terminus.
Cell surface detection of S1PR3 in Raji (human Burkitt's lymphoma) living cells: - ___Unstained cells.___Cells stained with Anti-S1PR3 (EDG3) (extracellular) Antibody(#ASR-013) (5 g/5x105cells).
quantity:
price:
to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: ATTO 488
application: flow cytometry 
Cell surface detection of S1PR3 in human Jurkat intact live cells: Cell surface detection of S1PR3 in human Jurkat intact live cells: Black line: Unstained cells. Green line: Cells + Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody (#ASR-013-AG), (5-10 µg/5x105 cells).
quantity:
price:
to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry 
Cell surface detection of S1PR3 in live intact mouse J774 macrophage cells: Cell surface detection of S1PR3 in live intact mouse J774 macrophage cells: Black line: Cells. Black line: Cells + rabbit IgG isotype control-FITC. Black line: Cells + Anti-S1PR3 (EDG3) (extracellular)-FITC Antibody (#ASR-013-F), (2.5 µg).
quantity:
price:
to the supplier - domestic rabbit monoclonal (JE53-79)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of EDG3 / S1P3 on different lysates with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/1,000 dilution. Lane 1: HepG2 cell lysate, 20 µg/Lane Lane 2: 293T cell lysate, 20 µg/Lane Lane 3: PC-12 cell lysate, 20 µg/Lane Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722419) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JE38-31)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation 
Western blot analysis of EDG3 / S1P3 on different lysates with Rabbit anti-EDG3 / S1P3 antibody (HA722337) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722337) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
EDG3 / S1P3 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA722337 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722337 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA722337 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA722337 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 minutes 20 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat S1pr3
- synonym:Edg3; Edg3l; lpb3
- description:sphingosine-1-phosphate receptor 3
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- S1pr3 antibody
linkout
questions and comments