rat S100a4 antibody | 1 product in Validated Antibody Database; 3 cited in the literature; 7 total from 6 suppliers

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Purified anti-S100A4
BioLegend
catalog: 810101

domestic rabbit polyclonal (S100A4)
reactivity: human, mouse, rat
application: immunocytochemistry, immunohistochemistry - paraffin section
citations: 3

quantity: 250 μL
price: 386 USD
to the supplier
S100A4 Antibody - BSA Free
Novus Biologicals
catalog: NBP1-89402

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3

quantity: 0.1 ml (also 25 ul)
price: 559 USD
to the supplier
Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free
Abcam
catalog: ab220213

domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg, 1 mg
price:
to the supplier
S100A4
HUABIO
catalog: ET1612-13-50UL

domestic rabbit monoclonal (SD200-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of S100A4 on different lysates with Rabbit anti-S100A4 antibody (ET1612-13) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of S100A4 on different lysates with Rabbit anti-S100A4 antibody (ET1612-13) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 7 hours add 300ng/mL BFA for last 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-13) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling S100A4 with Rabbit anti-S100A4 antibody (ET1612-13) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100A4 antibody (ET1612-13) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 50μl
price: 205.00 USD
to the supplier
S100A4
HUABIO
catalog: HA750282

domestic rabbit monoclonal (SD200-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of S100A4 on different lysates with Rabbit anti-S100A4 antibody (HA750282) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of S100A4 on different lysates with Rabbit anti-S100A4 antibody (HA750282) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 7 hours add 300ng/mL BFA for last 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750282) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling S100A4 with Rabbit anti-S100A4 antibody (HA750282) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100A4 antibody (HA750282) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
Goat anti-S100A4/CAPL Antibody
MyBioSource
catalog: MBS420725

domestic goat polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, enzyme immunoassay

quantity: 0.1 mg
price:
to the supplier
Anti-S100A4 antibody produced in rabbit
MilliporeSigma
catalog: HPA007973

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry
citations: 1

quantity:
price:
to the supplier