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- domestic rabbit monoclonal (PSH0-40)
reactivity: human, rat
application: western blot, flow cytometry
Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA721342) at 1/1,000 dilution. Lane 1: HeLa cell lysate (32 µg/Lane) Lane 2: HEK-293 cell lysate (33 µg/Lane) Lane 3: U87-MG cell lysate (30 µg/Lane) Lane 4: A549 cell lysate (26 µg/Lane) Lane 5: SH-SY5Y cell lysate (18 µg/Lane) Lane 6: PC-12 cell lysate (23 µg/Lane) Lane 7: L6 cell lysate (14 µg/Lane) Lane 8: Human cerebellum tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721342) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA721342) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RTN3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721342) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH0-40)
reactivity: human, rat
application: western blot, flow cytometry 
Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA750618) at 1/1,000 dilution. Lane 1: HeLa cell lysate (32 µg/Lane) Lane 2: HEK-293 cell lysate (33 µg/Lane) Lane 3: U87-MG cell lysate (30 µg/Lane) Lane 4: A549 cell lysate (26 µg/Lane) Lane 5: SH-SY5Y cell lysate (18 µg/Lane) Lane 6: PC-12 cell lysate (23 µg/Lane) Lane 7: L6 cell lysate (14 µg/Lane) Lane 8: Human cerebellum tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750618) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA750618) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RTN3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750618) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (HA750618) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (HA750618) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Rtn3
- description:reticulon 3
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- Rtn3 antibody
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