Your Filters
product
- domestic rabbit monoclonal (JE63-19)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of RSK2 on different lysates with Rabbit anti-RSK2 antibody (HA721033) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 84 kDa Observed band size: 75 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721033) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RSK2 on different lysates with Rabbit anti-RSK2 antibody (HA721033) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RSK2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 75 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721033) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH/3T3 cells labeling RSK2 with Rabbit anti-RSK2 antibody (HA721033) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RSK2 antibody (HA721033) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section 
Western Blot: RSK2 Antibody [NBP2-20237] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with RSK2 antibody and competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
quantity: 0.1 ml
price: 449 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal (D21B2)
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunocytochemistry, flow cytometry
citations: 6
gene information - rat Rps6ka3
- synonym:RGD1563860
- description:ribosomal protein S6 kinase A3
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Rps6ka3 antibody
questions and comments