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- domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot
citations: 1 - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
Figure 1. Western blot analysis of RNF43 using anti-RNF43 antibody (A01694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human 293T whole cell lysates,. Lane 3: human COLO-320 whole cell lysates,. Lane 4: human SW620 whole cell lysates,. Lane 5: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF43 antigen affinity purified polyclonal antibody (Catalog # A01694-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF43 at approximately 86KD. The expected band size for RNF43 is at 86KD.
quantity: 100µg/vial
price: 315 USD
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gene information - rat Rnf43
- synonym:RGD1305204
- description:ring finger protein 43
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product type
- Rnf43 antibody
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