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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
citations: 1
Western Blot: RGS6 Antibody [NB100-56659] - analysis of RGS6 in 30 ugs of mouse brain tissue lysate using this antibody.
quantity: 0.2 ml (also 0.05 ml)
price: 349 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of RGS6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500233, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate
Western blot analysis of RGS6 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500233, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Y79 cells labeling RGS6 with Rabbit anti-RGS6 antibody (HA500233) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RGS6 antibody (HA500233) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot
gene information - rat Rgs6
- description:regulator of G-protein signaling 6
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Rgs6 antibody
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