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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal (PSH0-15)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: C2C12 cell lysate Lane 4: 293 cell lysate Lane 5: MCF-7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: K562 cell lysate Lane 8: N2A cell lysate Lane 9: PC-12 cell lysate Lane 10: RAW264.7 cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721273) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution. Lane 1: NIH-3T3-si NT cell lysate Lane 2: NIH-3T3-si RbBP5 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. HA721273 was shown to specifically react with RbBP5 in NIH-3T3-si NT cells. Weakened band was observed when NIH-3T3-si RbBP5 sample was tested. NIH-3T3-si NT and NIH-3T3-si RbBP5 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721273, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH0-15)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA750585) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: C2C12 cell lysate Lane 4: 293 cell lysate Lane 5: MCF-7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: K562 cell lysate Lane 8: N2A cell lysate Lane 9: PC-12 cell lysate Lane 10: RAW264.7 cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750585) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA750585) at 1/1,000 dilution. Lane 1: NIH-3T3-si NT cell lysate Lane 2: NIH-3T3-si RbBP5 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. HA721273 was shown to specifically react with RbBP5 in NIH-3T3-si NT cells. Weakened band was observed when NIH-3T3-si RbBP5 sample was tested. NIH-3T3-si NT and NIH-3T3-si RbBP5 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721273, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of RBBP5 on different lysates with Rabbit anti-RBBP5 antibody (HA500395) at 1/1,000 dilution. Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate Lane 3: K562 cell lysate Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lane 8: N2A cell lysate Lane 9: RAW264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500395) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Rbbp5
- description:RB binding protein 5, histone lysine methyltransferase complex subunit
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product type
- Rbbp5 antibody
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