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- domestic rabbit monoclonal (JE65-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry - domestic rabbit monoclonal (JE65-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of RAB45 on different lysates with Rabbit anti-RAB45 antibody (HA721097) at 1/5,000 dilution. Lane 1: C6 cell lysate Lane 2: PC-12 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse spleen tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 1 minute 55 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721097) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RAB45 on different lysates with Rabbit anti-RAB45 antibody (HA721097) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: A431 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721097) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of PC-12 cells labeling RAB45 with Rabbit anti-RAB45 antibody (HA721097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAB45 antibody (HA721097) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
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gene information - rat Rasef
- description:RAS and EF hand domain containing
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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- Rasef antibody
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