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- mouse monoclonal (OTI11B2)
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1 - mouse monoclonal (OTI11B2)
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western Blot: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.
quantity: 100 ug
price: 579 USD
to the supplier - domestic rabbit monoclonal (5C10W9)
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (SC06-49)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of RALBP1 on different lysates with Rabbit anti-RALBP1 antibody (ET1610-88) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: COS-1 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 90 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-88) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RALBP1 on different lysates with Rabbit anti-RALBP1 antibody (ET1610-88) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RALBP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 76 kDa Observed band size: 90 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-88) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-RALBP1 antibody (ET1610-88) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-88) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
citations: 1
gene information - rat Ralbp1
- synonym:RIP1; RLIP76
- description:ralA binding protein 1
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Ralbp1 antibody
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