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- domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry
Western blot analysis of Rad51B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500010, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SKBR3 cell lysate Lane 2: PC-12 cell lysate
Flow cytometric analysis of SiHa cells labeling Rad51B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500010, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
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gene information - rat Rad51b
- synonym:RGD1560681; Rad51l1
- description:RAD51 paralog B
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product type
- Rad51b antibody
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