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- mouse monoclonal (5E9)
reactivity: human, mouse, rat
application: western blot
citations: 2
Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 (SMC-614). Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody (SMC-614). Courtesy of: Dario Alessi Lab, University of Dundee.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
application: WB. dilution: 1 : 1000, sample: pancreas homogenate
quantity: 50 µg
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of Rab3D on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500165, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse pancreas tissue lysate Lane 2: Rat pancreas tissue lysate Predicted band size: 24 kDa Observed band size: 24 kDa
Western blot analysis of Rab3D on PC-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500165, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 24 kDa Observed band size: 25 kDa
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Rab3D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500165, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Rab3d
- description:RAB3D, member RAS oncogene family
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product type
- Rab3d antibody
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