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- mouse monoclonal ([1543CT354.60.92])
reactivity: human, mouse, rat
application: western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section
Overlay histogram showing HepG2 cells stained with MBS9215444 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9215444, 1:25 dilution) for 60 min at 37 C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(at 1/400 dilution for 40 min at 37 C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-RAB3B Antibody at 1:2000 dilution. Lane 1: mouse brain lysate. Lane 2: rat brain lysate. Lane 3: SW480 whole cell lysate. Lane 4: human brain lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
quantity: 0.05 mL
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to the supplier - mouse monoclonal (1543CT354.60.92)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (EPR12987)
reactivity: human, mouse, rat
application: western blot, flow cytometry
gene information - rat Rab3b
- description:RAB3B, member RAS oncogene family
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product type
- Rab3b antibody
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