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- domestic rabbit monoclonal (JE66-08)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-RAB22A KD cell lysate (10 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: HeLa cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: Jurkat cell lysate (15 µg/Lane) Lane 6: U-2 OS cell lysate (15 µg/Lane) Lane 7: A549 cell lysate (15 µg/Lane) Lane 8: HEK-293 cell lysate (15 µg/Lane) Lane 9: Mouse brain tissue lysate (30 µg/Lane) Lane 10: Rat brain tissue lysate (30 µg/Lane) Lane 11: Mouse kidney tissue lysate (30 µg/Lane) Lane 12: Mouse testis tissue lysate (30 µg/Lane) Lane 13: Rat liver testis lysate (30 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of MCF7 cells labeling RAB22A with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - Anti-RAB22A antibody [EPR9487] - BSA and Azide free
Abcam
catalog: ab248852
total number of products: 4domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot
gene information - rat Rab22a
- description:RAB22A, member RAS oncogene family
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product type
- Rab22a antibody
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