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- domestic rabbit monoclonal (SC06-78)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Western Blot: HLA DR/DP Antibody (SC06-78) [NBP2-67610]
quantity: 100 ul
price: 449 USD
to the supplier - domestic rabbit monoclonal (SC06-78)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of HLA-DR on different lysates with Rabbit anti-HLA-DR antibody (ET1610-66) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling HLA-DR with Rabbit anti-HLA-DR antibody (ET1610-66) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HLA-DR antibody (ET1610-66) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of B16F1 cells labeling HLA-DR with Rabbit anti-HLA-DR antibody (ET1610-66) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HLA-DR antibody (ET1610-66) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 50μl
price: 205.00 USD
to the supplier - domestic rabbit monoclonal (SC06-78)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of HLA-DR on different lysates with Rabbit anti-HLA-DR antibody (HA750230) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750230) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling HLA-DR with Rabbit anti-HLA-DR antibody (HA750230) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HLA-DR antibody (HA750230) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of B16F1 cells labeling HLA-DR with Rabbit anti-HLA-DR antibody (HA750230) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HLA-DR antibody (HA750230) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (SC06-78)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section - mouse monoclonal (OX17)
reactivity: rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
gene information - rat RT1-Da
- synonym:DA1; H2-Ea; RT1-u
- description:RT1 class II, locus Da
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product type
- RT1-Da antibody
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