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- domestic rabbit monoclonal (JU60-33)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of PSME1 on different lysates with Rabbit anti-PSME1 antibody (ET7106-98) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: Rat lung tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-98) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PSME1 on different lysates with Rabbit anti-PSME1 antibody (ET7106-98) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PSME1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-98) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of PSME1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
gene information - rat Psme1
- description:proteasome activator subunit 1
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product type
- Psme1 antibody
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