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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of PSMD8 on different lysates with Rabbit anti-PSMD8 antibody (HA500408) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 30 kDa Exposure time: 5 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500408) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PSMD8 on Hela cell lysates with Rabbit anti-PSMD8 antibody (HA500408) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500408) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH/3T3 cells labeling PSMD8 with Rabbit anti-PSMD8 antibody (HA500408) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PSMD8 antibody (HA500408) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
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gene information - rat Psmd8
- description:proteasome 26S subunit, non-ATPase 8
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product type
- Psmd8 antibody
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