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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of PSMB7 on different lysates with Rabbit anti-PSMB7 antibody (HA500345) at 1/5,000 dilution. Lane 1: COS-1 cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: Rat testis tissue lysate (40 µg/Lane) Lane 5: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 30 kDa Observed band size: 27 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500345) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PSMB7 on different lysates with Rabbit anti-PSMB7 antibody (HA500345) at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500345) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-PSMB7 antibody (HA500345) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500345) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - mouse monoclonal (OTI1E1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section 
Western Blot: PSMB7 Antibody (OTI1E1) - Azide and BSA Free [NBP2-73645] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
quantity: 100 ug
price: 579 USD
to the supplier - mouse monoclonal (OTI1E1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
gene information - rat Psmb7
- description:proteasome 20S subunit beta 7
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Psmb7 antibody
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