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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2 - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of PSMB2 on different lysates with Rabbit anti-PSMB2 antibody (HA500410) at 1/10,000 dilution. Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: Hela cell lysate Lane 4: CAL62 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500410) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMB2 antibody (HA500410) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500410) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PSMB2 antibody (HA500410) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500410) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
gene information - rat Psmb2
- description:proteasome 20S subunit beta 2
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Psmb2 antibody
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