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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE07-47)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
AMPK gamma 1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722691 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722691 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722691 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722691 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801
quantity: 100μl
price: 385.00 USD
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gene information - rat Prkag1
- synonym:Prkaac; Prkga1
- description:protein kinase AMP-activated non-catalytic subunit gamma 1
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- Prkag1 antibody
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