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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit monoclonal (PSH15-92)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/20,000 dilution. Lane 1: NIH/3T3 cell lysate (negative) Lane 2: CTLL-2 cell lysate Lane 3: C2C12 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 75 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/10,000 dilution. Lane 1: Ramos cell lysate (negative) Lane 2: NK-92 cell lysate Lane 3: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Perforin-1 on rat spleen tissue lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/5,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 25 seconds ; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH15-92)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA751583) at 1/20,000 dilution. Lane 1: NIH/3T3 cell lysate (negative) Lane 2: CTLL-2 cell lysate Lane 3: C2C12 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 75 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751583) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA751583) at 1/10,000 dilution. Lane 1: Ramos cell lysate (negative) Lane 2: NK-92 cell lysate Lane 3: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751583) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Perforin-1 on rat spleen tissue lysates with Rabbit anti-Perforin-1 antibody (HA751583) at 1/5,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 25 seconds ; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751583) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: rat
application: western blot 
Western blot analysis of Jurkat cell lysate probed with Rabbit anti perforin antibody
quantity: 50 µg
price: 339 USD
to the supplier
gene information - rat Prf1
- synonym:Cyta; RATCYTA
- description:perforin 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Prf1 antibody
- Prf1 protein
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