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- domestic rabbit monoclonal (JE65-64)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of PREPL on different lysates with Rabbit anti-PREPL antibody (HA721069) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PREPL KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 70 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721069) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PREPL on different lysates with Rabbit anti-PREPL antibody (HA721069) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 70 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721069) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-PREPL antibody (HA721069) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721069) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free
Abcam
catalog: ab251361
total number of products: 4domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry - Anti-PPCEL/PREPL antibody [EPR17752] - BSA and Azide free
Abcam
catalog: ab251393
total number of products: 4domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
gene information - rat Prepl
- synonym:RGD1310143
- description:prolyl endopeptidase-like
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product type
- Prepl antibody
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