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- domestic rabbit monoclonal (SN0756)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of PPP1R1A on different lysates with Rabbit anti-PPP1R1A antibody (ET1611-60) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PPP1R1A on different lysates with Rabbit anti-PPP1R1A antibody (ET1611-60) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PPP1R1A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-60) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of PPP1R1A in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
gene information - rat Ppp1r1a
- description:protein phosphatase 1, regulatory (inhibitor) subunit 1A
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- Ppp1r1a antibody
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