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- domestic rabbit monoclonal (JM33-24)
reactivity: human, rat
application: western blot, immunoprecipitation, flow cytometry
Western blot analysis of PGC1 beta on different lysates with Rabbit anti-PGC1 beta antibody (ET1705-65) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 113 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PGC1 beta on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of PGC1 beta was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-65, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JF09-71)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/2,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lane 4: Human kidney tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Mouse heart tissue lysate Lane 10: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 91/113 kDa Observed band size: 91/113 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/1,000 dilution. Lane 1: His-tagged PGC1 alpha recombinant protein Lane 2: His-tagged PGC1 beta recombinant protein Lysates/proteins at 50 ng/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Ppargc1b
- synonym:PGC1beta; Perc
- description:PPARG coactivator 1 beta
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Ppargc1b antibody
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