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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of BOB1 on Daudi lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of BOB1 was done on Daudi cells. The cells were fixed, permeabilized and stained with BOB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
quantity: 100μl
price: 330 USD
to the supplier - mouse monoclonal (OTI2D4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - mouse monoclonal (OTI2D4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
gene information - rat Pou2af1
- description:POU class 2 homeobox associating factor 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Pou2af1 antibody
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