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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
Western blot analysis of PORCN on different lysates with Rabbit anti-PORCN antibody (HA500546) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: SiHa cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: SH-SY5Y cell lysate (20 µg/Lane) Lane 6: Neuro-2a cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: Mouse kidney tissue lysate (40 µg/Lane) Lane 9: Mouse brain tissue lysate (40 µg/Lane) Lane 10: Rat brain tissue lysate (40 µg/Lane) Lane 11: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 37 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500546) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
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gene information - rat Porcn
- synonym:RGD1564947
- description:porcupine O-acyltransferase
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product type
- Porcn antibody
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