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- domestic rabbit monoclonal (JM106-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Urokinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-26, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-3 cell lysate Lane 2: MCF-7 cell lysate
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Urokinase antibody (ET1703-26) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-26) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Urokinase antibody (ET1703-26) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-26) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, rat
application: western blot 
Western blot analysis of Urokinase on different lysates with Rabbit anti-Urokinase antibody (HA500429) at 1/2,000 dilution. Lane 1: PC-3M cell lysate (10 µg/Lane) Lane 2: MCF-7 cell lysate (10 µg/Lane) Lane 3: LANCAP cell lysate (10 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Lane 5: Rat stomach tissue lysate (20 µg/Lane) Predicted band size: 49 kDa Observed band size: 56 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500429) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of PC-3M cells labeling Urokinase with Rabbit anti-Urokinase antibody (HA500429) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-Urokinase antibody (HA500429) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, Red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (JM106-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section 
Western Blot: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]
quantity: 100 ul
price: 449 USD
to the supplier - mouse monoclonal (PGM2005)
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry - mouse monoclonal ([H77A1])
reactivity: mouse, rat
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section
gene information - rat Plau
- synonym:UPAM
- description:plasminogen activator, urokinase
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product type
- Plau antibody
- Plau protein
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