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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry - domestic rabbit monoclonal (JE65-06)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of PGM1 on different lysates with Rabbit anti-PGM1 antibody (HA721110) at 1/5,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: COS-1 cell lysate (20 µg/Lane) Lane 6: Mouse spleen tissue lysate (30 µg/Lane) Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721110) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PGM1 on different lysates with Rabbit anti-PGM1 antibody (HA721110) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PGM1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PGM1 on different lysates with Rabbit anti-PGM1 antibody (HA721110) at 1/1,000 dilution. Lane 1: 293T cell lysate, 10 µg/Lane Lane 2: A431 cell lysate, 10 µg/Lane Lane 3: Hela cell lysate, 10 µg/Lane Lane 4: HepG2 cell lysate, 10 µg/Lane Lane 5: NIH/3T3 cell lysate, 10 µg/Lane Lane 6: RAW264.7 cell lysate, 10 µg/Lane Lane 7: PC-12 cell lysate, 10 µg/Lane Lane 8: Rat spleen tissue lysate, 20 µg/Lane Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721110) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Pgm1
- description:phosphoglucomutase 1
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product type
- Pgm1 antibody
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