PELP1 Antibody (JE02-42)
Novus Biologicals
catalog: NBP3-32734

domestic rabbit monoclonal (JE02-42)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

PELP1 Antibody (JE02-42)
quantity: 100 ul
price: 449 USD
to the supplier

PELP1
HUABIO
catalog: HA722138

domestic rabbit monoclonal (JE02-42)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody (HA722138) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 120 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722138) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722138 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722138 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722138 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722138 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801

Immunocytochemistry analysis of MCF7 cells labeling PELP1 with Rabbit anti-PELP1 antibody (HA722138) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PELP1 antibody (HA722138) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Anti-PELP1 antibody [EPR15212] - BSA and Azide free
Abcam
catalog: ab250932

domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section

quantity: 100µg, 1 mg
price:
to the supplier

Anti-PELP1 antibody [EPR15212]
Abcam
catalog: ab187653

domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section

quantity: 100µL, 1 mL
price:
to the supplier