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- domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA, immunohistochemistry - paraffin section
Western blot analysis of lysates from Hela, Jurkat cell line (from left to right), using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Mouse Pak3 Antibody (N-term) (MBS9209950)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
quantity: 0.08 mL
price:
to the supplier - domestic rabbit monoclonal (SU03-70)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: SH-SY5Y cell lysate
ICC staining of Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JE57-39)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of PAK3 on different lysates with Rabbit anti-PAK3 antibody (ET7111-35) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: C6 cell lysate Lane 3: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-35) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - Anti-PAK1 (phospho S144) + PAK2 (phospho S141) + PAK3 (phospho S154) antibody [E ...
Abcam
catalog: ab40795domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
citations: 2
gene information - rat Pak3
- synonym:beta-PAK
- description:p21 (RAC1) activated kinase 3
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Pak3 antibody
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